Dzidza nezve Mini PCR: Chishandiso chekushandura chemocular biology

Kubva payakavambwa muma1980, iyo polymerase chain reaction (PCR) yakashandura ndima yemolecular biology. Iyi nzira inobvumira masayendisiti kukudza zvikamu zveDNA, zvichibvumira kuongororwa kwakadzama kwemajini. Pakati pekufambira mberi kwakasiyana-siyana muPCR tekinoroji, mini-PCR yabuda seimwe compact uye inoshanda imwe nzira inogona kusangana neyakasiyana maapplication mukutsvaga, kuongororwa, uye dzidzo.

Chii chinonzi Mini PCR?

Mini PCR michina, inowanzonzi mini thermal cyclers, idiki, inotakurika shanduro dzechinyakare PCR michina. Zvishandiso izvi zvakagadzirirwa kuita basa rimwechete rekutanga semichina mikuru yePCR: kukudza DNA. Nekudaro, mini PCR michina yakagadziridzwa kuti iite mavhoriyamu madiki emuenzaniso, kazhinji ari pakati pe5 uye 20 microliters, zvichiita kuti ive yakakodzera kune maapplication ane mashoma mashoma eDNA.

Michina yeMicroPCR idiki uye yakanyatsokodzera maLab ane nzvimbo shoma kana yebasa remumunda uko kutakurika kunodiwa. Michina mizhinji yeMicroPCR haina huremu uye inogona kufambiswa nemabhatiri, ichibvumira vaongorori kuita ongororo munzvimbo dziri kure kana kunze.

Zvishandiso zveMini PCR

1. Tsvagiridzo neKuvandudza: Munzvimbo dzekutsvagisa dzedzidzo uye dzeindasitiri, michina yemicroPCR inobatsira zvakanyanya patsvakiridzo yemajini, cloning, uye kutevedzana. Vatsvagiri vanogona kukurumidza kuyedza fungidziro nekusimudzira chaiyo DNA kutevedzana kuti vaongorore gene kutaura, shanduko, uye genetic kusiyana.

2. Kuongororwa: Mini-PCR iri kuwedzera kushandiswa mukuongororwa kwekliniki, kunyanya mukuongororwa kwechirwere chinotapukira. Semuenzaniso, panguva yeCOVID-19 denda, kukurumidza kuyedzwa kwave kwakakosha, uye mini-PCR zviridzwa zvinofambisa kukurumidza kukwidziridzwa kwehutachiona RNA, zvichibvumira kuongororwa uye kurapwa panguva. Zvichienzaniswa nemaitiro echinyakare, ivo vanokwanisa kupa mhedzisiro munguva pfupi, zvichiita ivo sarudzo yekutanga kune akawanda emakiriniki marabhoritari.

3. Dzidzo: Mini PCR michina iri kuwanawo nzira yavo muzvikoro zvedzidzo. Vanopa vadzidzi maoko-pane ruzivo nemamolekyuli biology matekiniki, zvichivabvumira kuti vanzwisise misimboti yeDNA kukwidziridzwa uye kuongororwa. Hukuru hudiki uye dhizaini-inoshamwaridzika yezvishandiso izvi zvinoita kuti zvive zvakanaka kuti zvishandiswe mukirasi, zvichibvumira vadzidzi kuita zviedzo pasina kudiwa kwenzvimbo huru yerabhoritari.

4. Kutariswa Kwezvakatipoteredza: Musainzi yezvakatipoteredza, zviridzwa zvemicroPCR zvinoshandiswa kuona uye kuyera huwandu hwehutachiona hwehutachiona munzvimbo dzakasiyana siyana. Vatsvaguri vanogona kuongorora ivhu, mvura, uye masampu emhepo nekuda kwekuvapo kwehutachiwana chaihwo kana zviratidzo zvehutano hwezvakatipoteredza. Chishandiso ichi chakanyanya kukosha pakuongorora kukonzeresa kusvibiswa kwemamiriro ekunze uye kushanduka kwemamiriro ekunze pane zvipenyu zvakasiyana-siyana.

5. Forensic Sayenzi: Mukuferefeta kweforensic, michina midiki yePCR inoita basa guru mukuongorora humbowo hweDNA panzvimbo dzine mhosva. Ivo vanokwanisa kukwidziridza huwandu hweDNA, vachibvumira vezvesainzi vezvesainzi kuti vagadzire maprofile kubva kuhumbowo hwekutsvagira, nekudaro vachibatsira kuferefeta kwemhosva uye kutongwa kwemutemo.

mukupedzisa

Mini-PCR inomiririra kufambira mberi kukuru mumunda wemolecular biology, ichipa chishandiso chakasiyana-siyana, chinoshanda chekushandisa kwakasiyana siyana. Kutakurika kwayo, nyore kushandisa, uye kugona kugadzira masampuli madiki kunoita kuti ive chishandiso chakakosha kune vanotsvaga, varapi, vadzidzisi, uye masayendisiti ezvemamiriro ekunze. Sezvo tekinoroji ichiramba ichifambira mberi, mini-PCR ingangoita basa rinowedzera kukosha mukusimudzira kunzwisisa kwedu genetics nekuvandudza kugona kwekuongorora mundima dzakawanda. Kungave murabhoritari, kirasi, kana munda, mini-PCR inosimudzira nzira yatinodzidza nayo molecular biology uye mashandisiro ayo mazhinji.