Emkhakheni webhayoloji yamangqamuzana, i-PCR (Polymerase Chain Reaction) iguqule indlela esenza ngayo ukuhlola kofuzo, ukuxilonga, kanye nocwaningo. Ngokukhuphuka kwe PCR encane imishini, isimo sobuchwepheshe be-PCR sesishintshile, sivumela ukuphatheka, ukufinyeleleka, nokufinyeleleka. Lesi sihloko siyahlola PCR encane imishini, equkethe abaholayo Abakhiqizi bemishini ye-PCR nokugqamisa ukubaluleka kokuhlolwa kwe-PCR ezifuyweni, ikakhulukazi amakati.
Uma kuziwa ekuthuthukisweni kwe PCR encane imishini, eminingana Abakhiqizi bemishini ye-PCR baqhamuke njengamavulandlela kulo mkhakha. Izinkampani ezifana ne-Thermo Fisher Scientific, Bio-Rad, ne-Qiagen zihamba phambili, zikhiqiza imishini ye-PCR ehlangene, esezingeni eliphezulu ebhekelela kokubili izikhungo zocwaningo nemitholampilo yezilwane. Laba bakhiqizi bazibophezele ekusunguleni izinto ezintsha, baqinisekisa ukuthi imikhiqizo yabo isebenziseka kalula futhi iyakwazi ukuletha imiphumela ethembekile ngokushesha.
I-PCR encane imishini yakhelwe ukunikeza ukunemba nokuthembeka okufanayo njengozakwabo abakhudlwana kodwa ngosayizi ohlangene olingana kalula nezilungiselelo ezihlukahlukene. Ukusebenziseka kwazo kalula nokusebenza kahle kwazo kuzenza zilungele amalabhorethri anendawo elinganiselwe noma kubacwaningi abenza umsebenzi wasendle. Ngokusekelwa ngabahloniphekile Abakhiqizi bemishini ye-PCR, PCR encane ubuchwepheshe buya ngokuya bufinyeleleka kochwepheshe emikhakheni eyahlukene.
Enye yezinzuzo ezibaluleke kakhulu ze PCR encane ubuchwepheshe wukuvela kwemishini eshibhile ye-PCR. Lezi zixazululo ezithengekayo ziguqula indlela amalabhorethri nemitholampilo esebenza ngayo, okubenza bakwazi ukwenza izivivinyo ezibalulekile ngaphandle kokwephula imali. Kubacwaningi, othisha, kanye nemitholampilo yezilwane, umshini we-PCR obiza kancane ungasho umehluko phakathi kokwenza ukuhlolwa okubalulekile kanye nokuvinjelwa yizingqinamba zesabelomali.
I-PCR encane imishini akuwona nje ukonga kodwa futhi yakhelwe ukusebenza kahle. Banikeza imijikelezo esheshayo yokushisisa nokupholisa, enciphisa sonke isikhathi esidingekayo kuzinqubo ze-PCR. Ngenxa yalokho, amalabhorethri angakwazi ukwandisa ukusebenza kwawo ngenkathi egcina imiphumela yekhwalithi ephezulu. Lokhu kunenzuzo ikakhulukazi emitholampilo emincane nasezikhungweni zocwaningo ezidinga okokusebenza okuthembekile ngaphandle kwenani elishisiwe.
Njengabantu, abangani bethu bezilwane bangazuza ekuhlolweni kokuxilonga okuthuthukile, futhi ukuhlolwa kwe-PCR kuhamba phambili emithini yezilwane. Ukuhlolwa kwe-PCR kwamakati kuyithuluzi elinamandla elisetshenziswa ukuthola ubukhona bamagciwane athile, ukuguqulwa kofuzo, nezifo ezithathelwanayo. Le ndlela yokuhlola yamangqamuzana inikeza imiphumela enembayo nesheshayo, iyenze ibe insiza ebaluleke kakhulu kodokotela bezilwane.
Ukuhlolwa kwe-PCR kwamakati kungasetshenziswa ukuxilonga izimo ezihlukahlukene, okuhlanganisa izifo ezibangelwa amagciwane afana ne-Feline Immunodeficiency Virus (FIV) ne-Feline Leukemia Virus (FeLV), kanye nezifo ezibangelwa amagciwane kanye nezifo ezithile zofuzo. Ngokwamukelwa kwe PCR encane imishini emisebenzini yezilwane, odokotela bezilwane bangenza lezi zivivinyo endlini, okuholela ekuxilongweni okusheshayo nokwelashwa okufika ngesikhathi kubangane bethu abanoboya.
Ukufika kwe PCR encane imishini iphawula ingqopha-mlando ebalulekile emhlabeni wokuhlolwa kwamangqamuzana. Ngokusekelwa ngabanegama elihle Abakhiqizi bemishini ye-PCR kanye nokuba khona kwemishini ye-PCR eshibhile, kokubili amalabhorethri kanye nemitholampilo yezilwane kungathuthukisa amakhono abo okuxilonga. Ngaphezu kwalokho, ukuqonda kokuhlolwa kwe-PCR kwamakati kugcizelela ukubaluleka kobuchwepheshe obuthuthukile ekuqinisekiseni impilo nokuphila kahle kwezilwane ezifuywayo.
Joyina uguquko kubuchwepheshe be-PCR futhi utshale imali esikhathini esizayo sokuhlola namuhla! Izwa izinzuzo ze PCR encane imishini futhi uqinisekise ukuthi unamathuluzi adingekayo ukuze wenze izinqumo ezinolwazi, noma ngabe kusendaweni yocwaningo noma umtholampilo wezilwane. Ungaphuthelwa yithuba lokuthuthukisa amakhono akho ngezisombululo zakamuva ze-PCR ezingabizi kakhulu, ezisebenza kahle.